| Title | Bio-Normalizer Modulates Free Radicals in Brain, Blood and Macrophage |
|---|---|
| Year | 1994 |
| Author | J. A. Osato; L.G. Korkina; L.A. Santiago; I.B. Afanas’ev; K.Horistu; A. Mori |
| Publisher | 7th Biennial Scientific Meeting |
SPONSORS
The Organising Committee thanks the following organisations and acknowledges their commitment to continuing education, research and the well-being of the community.
The Heart Research Institute,
Sydney Astra Hassle AB
Bioxytech SA
Daiichi Pharmaceutical Co Limited
Qantas Airways Limited
AIDAB
The Australian Tourist Commission
Blackmores Limited
Free Radical Sciences, Inc
Goodman Fielder Limited
Henkel Australia Pty Limited
International Science Foundation
Malaysian Palm Oil Promotion Council
Nikken Foods Company Ltd
Roche Vitamins & Fine Chemicals
Astra Pharmaceuticals
Beckman Instruments
Randox Laboratories Ltd
Alaris America
AMRAD Corporation
National Heart Foundation of Australia
Smoking and Health Research Foundation of Australia
Australian Society for Biochemistry and Molecular Biology Inc
SFRR (Australasia)
Betatene Limited
DITARD
ACKNOWLEDGEMENTS
Front cover Sydney Opera House photography by Don McMurdo,
courtesy of the Sydney Opera House
Front cover Aboriginal painting “Witchetty Grub Dreaming 1991″
by Paddy Japalijarri Sims
Inside back cover map courtesy of Sydney Convention and Exhibition Centre
Back cover photographs from the Australian Tourist Commission
WELCOME TO ISFRR ’94
On behalf of the International Society for Free Radical Research and the Organizing Committee of the 7th Biennial Scientific Meeting of the Society, we welcome you to Sydney and this conference.
We believe you will find the scientific programme stimulating and provocative, unusual in places, and of the highest quality. We look forward to the mutual exchanges which will result and hope you enjoy the programme.
We are grateful for the corporate support we have received and acknowledge the importance of such participation to the overall success of ISFRR ’94 and give special thanks to our sponsors for their commitment.
We welcome you and thank you for your participation and contribution to the success of ISFRR ’94.
Roger Dean
Chairman, ISFRR ’94
Heart Research Institute
145 Missenden Road Camperdown, Sydney NSW 2050 Australia
gold card sponsor of the conference
ISFRR ’94 ORGANISING COMMITTEE
Sydney Free Radical Group, Inc
Chairma
Roger Dean, Director
Heart Research Institute, Sydney
Secretary & Scientific Programme
Roland Stocker, Group Leader
Heart Research Institute, Sydney
Treasurer
Peter Southwell-Keely, Associate Professor
Department of Organic Chemistry
University of New South Wales
Vice-Chairperson
Dana Jamieson, Associate Professor
School of Physiology & Pharmacology
University of New South Wales
ISFRR ’94 SUB-COMMITTEE
Publicity and Promotion
Wendy Jessup, Group Leader
Heart Research Institute, Sydney
Social
Lorraine Assenza Heart Research Institute, Sydney
Sponsorship
David Sullivan Royal Prince Alfred Hospital, Sydney
Sub-Committee Members include
Mark Baker Phil Bone Jan Gebicki Sylvia Gebicki John Hodder Nick Hunt
Michael Perry Jeremy Simpson Jane Taylor Christine Winterbourn
CONFERENCE ORGANISER
Margaret Blackwell
Abacus Management Pty Limited
SFRR INTERNATIONAL COMMITTEE
President
Irwin Fridovich
President Elect
Robin Willson
Secretary-General
Lester Packer
Treasurer
Clemens von Sonntag
Representatives
Kelvin Davies
Oxygen Society
Roger Dean
SFRR Australasia
Bruce Freeman
Oxygen Society
Masayasu Inoue
SFRRAsia
Hiroe Nakazawa
SFRR Asia
Sten Orrenius
SFRR Europe
Guiseppe Poli
SFRR Europe
Christine Winterbourn
SFRR Australasia
Secretaries
John E Biaglow
The Oxygen Society
University of Pennsylvania School of Medicine Department
of Radiation Oncology 122 Levy Oral Health Building 4010 Locust Philadelphia, \
PA 19104-6002 U.S.A.
Victor Darley-Usmar
SFRR Europe
Biochemical Sciences The Wellcome Research Laboratories Langley Court
Beckenham Kent BR3 3BS United Kingdom
Wendy Jessup
SFRR Australasia
Heart Research Institute 145 Missenden Road Camperdown Sydney,
NSW 2050 Australia
Toshikazu Yoshikawa
SFRR Asia
Kyoto Prefectural University of Medicine First Department of Medicine Kamigyo-ku, Kyoto 602 Japan
SCIENTIFIC PROGRAMME
PL. A Radicals and Life Style Bruce Ames
PL.D Antioxidant Drug Targeting
Anthony Allison
PL.E Free Radicals and Metal Catalysis
Shosuke Kawanishi
PL.H Myocardial and Other Ischaemia and/or Reperfusion
David Hearse
PL.I Oxidative Reactions and Heme Proteins
Paul Ortiz de Montellano
PL.K Reactive Nitrogen Intermediates
Joseph Beckman
PL.M Particles and Pollutants
Brooke Mossman
PL.N DNA and Radicals
Sten Steenken
PL.R Free Radical Cytotoxicity
Christine Winterboum
PL.T Disorders of the CNS and Ageing
Robert Floyd
Conference Dinner Speech
Free Radical Mechanisms in Tissue Injury
Mario Dianzani
Sub-Plenary Session (SP)
SP.A Radicals and Life Style Lester Packer
SP.B Redox-active Protein and Carbohydrate Components
John Baynes
SP.C Radical and Antioxidant Reactions in Multi-Phase Systems
Keith Ingold
SP.D Antioxidant Drug Targeting John Eaton
SP.E Free Radicals and Metal Catalysis
to be announced
SP.F Oxidation and Antioxidation in Food Karen Schaich
SP.G Radicals/Oxidants and Arachidonic Acid Metabolism
Alvin Chan
SP.H Myocardial and Other Ischaemia and/or Reperfusion
Gregory Bulkley
SP.I Oxidative Reactions and Heme Proteins Tony Kettle
SP.J Enzymatic Defences against Oxidative Damage Stefan Marklund
SP.K Reactive Nitrogen Intermediates
Brad McDonald
SP.L Atherosclerosis Wendy Jessup
SP.M Particles and Pollutants Ann Aust
SP.O Oxidative Events in Proliferation and Replication
Nicholas Hunt
SP.N DNA and Radicals Nancy Oleinick
SP.P Inflammation Masayasu Inoue
SP.Q Spin Traps in Biomedicine
Michael Davies
SP.S Oxidants and Gene Expression Rex Tyrrell
SP.R Free Radical Cytotoxicity
Sten Orrenius
SP.T Disorders of the CNS and Ageing
Richard Cutler
Daiichi Lunch Session/Ebselen
01 Ebselen Transport and its LDL-Cholesterylester Hydroperoxide
Reducing Activity in Plasma
Helmut Sies
02 Inhibition of Oxidative Modification of LDL by Ebselen
Etsuo Niki
03 Status of Ebselen Development in Stroke TakaoAsano
F/G 7 DISTRIBUTION AND BILIARY EXCRETION OF
NATURAL AND UNNATURAL α -TOCOPHEROLS
Ichikawa H, Tadahiko U, Kiyose C, Igarashi 0
Tokyo Mtr. R/Lab. Pub. Health Tokyo, Japan Ochanomizu University, Tokyo. Japan
The present study was designed to investigate the biodiscrimination between natural and unnatural form of α- Tocopherol ( α – Toc) by feeding non-labeled 2-ambo-α-Toe Ac(equimolar mixture of RRR- (natural) and SRR-α-Toc Acs (unnatural)), which have no – possibility of hydrogen exchange and isotopic effects, to male rats.
The animals (F344/DuCrj, at the 4th week after birth) were fed diets containing 100 mg of 2 – ambo-α-Toc Ac/kg diet for 8 weeks. Amounts of α-Tocs in blood, tissues and bile were determined by newly developed HPLC method.
There were evident differences between the amounts of RRR- and SRR-α-Tocs in blood, tissues and bile. The amounts of SRR were 8.1% (plasma), non-detectable (ND, RBC), less than 15.7% (liver, or less (the others)). Besides, it was especially noted that the amounts of SRR in brain were ND.
And the excretion of RRR in bile was higher than that of SRR, and the amount of absorption of SRR via portal vein was very small.
F/G 9 BIO-NORMALIZER MODULATES FREE RADICALS
IN BRAIN, BLOOD AND MACROPHAGE
Osato JA1,2, Afanas’ev IB3, Korkina LG4, Santiago LA1,5, Horitsu H2 and Mori A5
1Osato Research Institute, Gifu, Japan; 2The United Graduate Sch. of Agricultural Science, Gifu University, Gifu, Japan; 3Vitamin E Research Institute, Moscow, Russia;4Russian Institute for Pediatric Hematology, Moscow, Russia; and 5Department of Neuroscience, Okayama University Medical School, Okayama, Japan
To provide for the scientific basis of the purported therapeutic and preventive actions of Bio-normalizer (BN), a fermented functional health food from papaya, we studied by electron spin resonance/spin trapping and chemiluminescence (CL) methods its effects on the free radical production in different systems. BN inhibited hydroxyl, peroxyl, carbon-centered, and lipid peroxides in various rat brain regions; suppressed oxygen radicals
in cell -free systems such as Fenton reaction, xanthine oxidase, H202 + NaCIO, H202 + horseradish peroxidase; reduced spontaneous and menadione induced superoxide release from human erythrocytes; decreased luminol-amplified CL but increased lucigeno-independent CL; and enhanced superoxide dismutase activity in inflammed murine macrophage. While BN prevented the formation of hyliroxyl and peroxyl radicals, it induced the production of intracellular superoxide radical by dormant and activated phagocytes, human neutrophils, and rat peritoneal macrophage.
THE EFFECTS OF α– AND δ -TOCOPHEROLS ON
F/G8 LIPID PEROXIDE FORMATION IN RAT TISSUES
Hirahara F. and Kimura S*.
Division of Food Sience, National Institute of Health and Nutrition, Graduate School of Nutrition, Showa
Women’s University*, Tokyo, Japan
Much work has been done to examine the antioxidant effects of tocopherol (toc) homologues in vitro. The present study was undertaken on the effects of α– or Ȣ – toc on lipid peroxide (LPO) formation in rat tissues. Hale Wistar strain rats 3 weeks old were acclimatized to a control diet for one week, and they were divided into 3 groups of six rats each. Group 1 was fed a vitamin E deficient diet; groups 2 and 3 were fed 100g of vitamin
E-deficient diet supplemented with 10mg of – or Ȣ – toc, respectively. Al l groups of rats were maintained on these diets for 3 weeks. The toe levels of serum and of tissues were determined by HPLC method. LPO values in the rat tissues were compared by the TBA and Chemiluminecence (CL) methods. α –Toc was widely distributed in rat t issues. A Ȣ-Toc content equal to that of the α-toc group was admitted into the adipose tissues, but trace amounts of Ȣ-toc in group 3 were admitted into other tissues. In group 3, LPO values (TBARS) of tissues were lower than in group I, but even the adipose tissues containing Ȣ-toc were much higher values than those of group 2.
Although only small amounts of Ȣ-toc was contained, in the testes and brain the low LPO values were admitted.
The LPO values (CLvalues) of the liver and testes in group were 2 >>3 > 1
BIO-NORMALIZERMODULATES FREE RADICALS IN BRAIN, BLOOD AND MACROPHAGE
OsatoJA1 2, Afanas‘ev IB3– Korkina LG4, Santiago LA1 5, Horitsu H22 andMoriA5
1Osato Research Institute
2The United Graduate Sch. of Agricultural Science, Gifu University
3Vitamin Research Institute, Moscow, Russia
4Russian Institute for Pediatric Hematology, Moscow, Russia
5Department of Neuroscience, Okayama University Medical School Okayama
Journal of Free Radicals in Biology & Medicine Volume 2 Number 3 (F/G9):1994
To provide for the scientific basis of the purported therapeutic and preventive actions of Bio-normalizer (BN), a fermented functional health food from papaya, we studied by electron spin resonance/spin trapping and chemiluminescence (CL) methods its effects on the free radical production in different systems. BN inhibited hydroxyl, peroxyl, carbon-centered, and lipid peroxides in various rat brain regions; suppressed oxygen radicals in cell-free systems such as Fenton reaction, xanthine-xanthine oxidase, H2O2+NaClO, H2O2+horseradish peroxidase; reduced spontaneous and menadione-induced superoxide release from human erythrocytes; decreased luminol-amplified CL but increased lucigenin-dependent CL; and enhanced superoxide dismutase activity in inflamed murine macrophage. While BN prevented the formation of hydroxyl and peroxyl radicals, it induced the production of intracellular superoxide radical by dormant and activated phagocytes, human neutrophils, and rat peritoneal macrophage.