| Title | Antioxidant Action of Bio-Normalizer on Asbestos- and Cobalt- Induced Lung Injury |
|---|---|
| Year | 1995 |
| Author | J. A. Osato; I.B. Afanas’ev; K. Takamizawa; L.G. Korkina |
| Publisher | Oxygen Club of California Annual Meeting, |
ANTIOXIDANT ACTION OF BIO-NORMALIZERON ASBESTOS
AND COBALT-INDUCED LUNG INJURY
J.A. Osato 1, 2, I. B. Afanas’ev 3, L. A. Santiago l, K. Takamizawa 2 & L.G. Korkina 4
1OSATO RESEARCH INSTITUTE, 2THE UNITED GRADUATE SCHOOL OF AGRICULTURAL SCIENCE, 3VITMIN RESEARCH INSTITUTE, 4RUSSIAN MEDICAL UNIVERSITY, LABORATORY OF OCCUPATIONAL LUNG DISEASES
Presented at OXYGEN CLUB OF CALIFORNIA Annual Meeting- Oxidants & Antioxidants in Biology ”Novel Antioxidants and Health Effects of Antioxidants Nutrients Oxidative Stress and Signal Transudation”
22-24 March 1995, San Francisco, California, USA
We have previously demonstrated in in vivo experiments that asbestos fibers and cobalt ions stimulate ROS (reactive oxygen species) production by neutrophils and macrophages hence causing inflammation and lung injury. To elucidate the antioxidant action of Bio-normalizer (BN) on asbestos- and cobalt-induced lung inflammation, two groups of Wistar rats were given intratracheal administration of 15 mg of chrysotile asbestos and 2.5mM of CoSO4, respectively, combined with 10-50 mg/ml of BN. BN administration substantially ameliorated the inflammation and fibrosis of lung tissue by free radical-mediated lung injury caused by lipid peroxidation and over production of ROS by pulmonary phagocytes post-induction with asbestos and cobalt. Analyses revealed that BN inhibited OH production, enhanced reduced GSH, and stimulate SOD activities in either RBC or WBC and macrophage. These findings show that BN may be an important anti-inflammatory and anti-fibrotic agent.
OXYGEN CLUB OF CALIFORNIA ANNUAL MEETING OXIDANTS & ANTIOXIDANTS IN BIOLOGY NOVEL ANTIOXIDANTS AND HEALTH EFFECTS OF ANTIOXIDANT NUTRIENTS OXIDATIVE STRESS AND SIGNAL TRANSDUCTION
22 – 24 March 1995 Sir Francis Drake Hotel San Francisco, California
BOOK OF ABSTRACTS
ANTIOXIDANT ACTION OF BIO-NORMALIZER ON ASBESTOS-
AND COBALT- INDUCED LUNG INJURY
J.A. Osato1.2, I.B. Afanas’ev3, L.A. Santiago1, K. Takamizawa2 & L.G. Korkina4
1OSATO RESEARCH INSTITUTE, 12- MINAMIMACHI, BAIRIN, GIFU 500 JAPAN
2THE UNITED GRADUATE SCHOOL OF AGRICULTURAL SCIENCE, GIFU UNIVERSITY,
1-1 Y ANAGIDO, GIFU 501-1 JAPAN
3VITAMIN RESEARCH INSTITUTE, MOSCOW 117680, RUSSIA
4RUSSIAN MEDICAL UNIVERSITY, LABORATORY OF OCCUPATIONAL LUNG DISEASES,
MOSCOW 117437, RUSSIA
We have previously demonstrated in in vivo experiments that asbestos fibers and cobalt ions stimulate ROS production by neutrophils and macrophages hence causing inflammation and lung injury. To elucidate the antioxidant action of Bio-normalizer (BN) on asbestos and cobalt-induced lung inflammation, two groups of Wistar rats were given intratracheal administration of 15 mg of chrysotile asbestos and 2.5 mM of CoSO4, respectively, combined with 10-50 mg/ml of BN. BN administration substantially ameliorated the inflammation and fibrosis of lung tissue by suppressing free radical-mediated lung injury caused by lipid peroxidation and over production of ROS by pulmonary phagocytes post-induction with asbestos and cobalt. Analyses revealed that BN inhibited ·OH production, enhanced reduced GSH, and stimulated SOD activities in either RBC or WBC and macrophage. These findings show that BN may be an important anti-inflammatory and anti-fibrotic agent.
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